Annotated protein:Gamma-aminobutyric acid receptor subunit alpha-3 (GABA(A) receptor subunit alpha-3). Gene symbol: GABRA3. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P20236
antibody wiki:
SynGO gene info:SynGO data @ GABRA3
Ontology domain:Cellular Component
SynGO term:synapse (GO:0045202)
Synapse type(s):thalamus, GABAergic
Annotated paper:Eyre MD, et al. "Setting the time course of inhibitory synaptic currents by mixing multiple GABA(A) receptor alpha subunit isoforms" J Neurosci. 2012 Apr 25;32(17):5853-67 PMID:22539847
Annotation description:This paper shows that mIPSCs in nucleus reticularis thalami (nRT) neurons are mediated by alpha3-containing GABA-A-Rs. The paper uses a combination of channel properties finger printing (channel decay kinetics), protein expression/localization to synapses, and a pharmacological agent (zolpidem) that in the given concentration and gene expression distinguishes alpha1- from alpha3-containing GABA-A-Rs. Only alpha1-containing GABA-A-Rs have sensitivity to zolpidem at the used concentration.

Fig.4: Co-localization with gephyrin (marker protein of GABA/Glycine postsynaptic specialization) is shown.
Evidence tracking, Biological System:Intact tissue
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Confocal
Annotator(s):Pim van Nierop (ORCID:0000-0003-0593-3443)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:2073
Dataset release (version):20210225
View annotation as GO-CAM model:Gene Ontology