| Annotated protein: | IQ motif and SEC7 domain-containing protein 2. Gene symbol: IQSEC2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q5DU25 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ IQSEC2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density, intracellular component (GO:0099092) |
| Synapse type(s): | brain, glutamatergic hippocampus, glutamatergic cerebellum, glutamatergic |
| Annotated paper: | Sakagami H, et al. "IQ-ArfGEF/BRAG1 is a guanine nucleotide exchange factor for Arf6 that interacts with PSD-95 at postsynaptic density of excitatory synapses" Neurosci Res. 2008 Feb;60(2):199-212 PMID:18164504 |
| Figure(s): | Fig. 6, 7, 8, 9 |
| Annotation description: | IQ-ArfGEF/BRAG1/IQSEC2 is present in the synaptic PSD fraction (Fig.6E) and interacts with PSD-95 (Fig. 6B, C, F) in in vitro-experiments and in homogenates from adult mouse forebrains. IQSEC2 immunoreactivity co-localizes with the labelling of PSD-95, a component of the postsynaptic density of asymmetric, excitatory synapses, but not with that of GABAARbeta1, indicating a specific localization of IQSEC2 to excitatory synapses (Fig.7, 8). Postembedding-immunogold labelling for electron microscopy confirms the presence of IQSEC2 at postsynaptic densities at asymmetrical synapses (Fig. 9). Rabbit antibodies were generated in this study using GST and MBP fusion proteins of the C-terminal region (116 aas) of IQSEC2. The specificity of the antibody was tested by Western Blot (Fig 6D) and using antibody, pre-absorved with the antigen, for IHC (data not shown). |
| Evidence tracking, Biological System: | Intact tissue Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal Western blot Biochemical fractionation (generic) IP + WB/MSMS |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 1053 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |