| Annotated protein: | Syntaxin-1A (Neuron-specific antigen HPC-1) (Synaptotagmin-associated 35 kDa protein) (P35A). Gene symbol: STX1A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P32851 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ STX1A |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of synaptic vesicle membrane (GO:0030285) |
| Synapse type(s): | brain |
| Annotated paper: | Walch-Solimena C, et al. "The t-SNAREs syntaxin 1 and SNAP-25 are present on organelles that participate in synaptic vesicle recycling" J Cell Biol. 1995 Feb;128(4):637-45 PMID:7860636 |
| Figure(s): | Fig. 2-5 |
| Annotation description: | The authors demonstrate that major pool of syntaxin 1 recycle with synaptic vesicles (SVs). Immunoisolation of SVs with synaptophysin and syntaxin 1 specific antibodies show that the most of the SVs contain syntaxin 1. Furthermore, the data suggests that syntaxin 1 is an integral membrane component of SVs (Fig. 3) The biochemical findings are confirmed by immunogold electron microscopy on purified SVs (Fig. 5) 2/9/2017 Pim - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Western blot Biochemical fractionation (generic) IP + WB/MSMS |
| Annotator(s): | Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498) |
| Lab: | Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
| Additional literature: | By application of cryoimmunogold labelling on rat cerebellum sections, Koh et al demonstrate that HPC-1 is localized on plasma membrane and synaptic vesicles of presynaptic boutons which form synapses with Purkinje cells (Fig. 3 and Fig. 11). @ PMID:8301329 Miya et al demonstrate the localization of syntaxin -1 to presynaptic nerve terminal plasma membrane and SVs (fig. 4) by immunoelectron microscopy. @ PMID:9078410 |
| SynGO annotation ID: | 1162 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |