Annotated protein:Intersectin-1 (EH domain and SH3 domain regulator of endocytosis 1). Gene symbol: ITSN1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9WVE9-1
antibody wiki:
SynGO gene info:SynGO data @ ITSN1
Ontology domain:Biological Process
SynGO term:regulation of modification of postsynaptic actin cytoskeleton (GO:1905274)
Synapse type(s):hippocampus, glutamatergic
Annotated paper:Thomas S, et al. "Intersectin regulates dendritic spine development and somatodendritic endocytosis but not synaptic vesicle recycling in hippocampal neurons" J Biol Chem. 2009 May 1;284(18):12410-9 PMID:19258322
Figure(s):fig 7, fig 8
Annotation description:In fig 7, the authors show that intersection 1-long isoform co-localizaes with actin in the post-synapse. In fig 7 they demonstrate that knockdown of intersection 1-long using lentivirus disrupts the development of dendritic spines.

15/8/2017 Pim
- It is important to note that: "Intersectin-l is a GEF for Cdc42. In vitro, intersectin-l stimulates the formation of branched actin filaments by activating Cdc42 with subsequent downstream activation of N-WASP (23)." While the paper shows only an effect on spine maturation, the inference is that this is via 'regulation of modification of postsynaptic actin cytoskeleton (GO:1905274)'
Evidence tracking, Biological System:Cultured neurons
Evidence tracking, Protein Targeting:RNAi / shRNA
Evidence tracking, Experiment Assay:Confocal
Annotator(s):Peter McPherson (ORCID:0000-0001-7806-5662)
Lab:Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada
Additional literature:Intersectin-l is a GEF for Cdc42. In vitro, intersectin-l stimulates the formation of branched actin filaments by activating Cdc42 with subsequent downstream activation of N-WASP. @ PMID:11584276
SynGO annotation ID:1179
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology