Annotated protein:	Syntaxin-binding protein 5 (Lethal(2) giant larvae protein homolog 3) (Tomosyn-1). Gene symbol: STXBP5. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9WU70
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SynGO gene info:	SynGO data @ STXBP5
Ontology domain:	Biological Process
SynGO term:	synaptic vesicle cycle (GO:0099504)
Synapse type(s):	hippocampus
Annotated paper:	Cazares VA, et al. "Dynamic Partitioning of Synaptic Vesicle Pools by the SNARE-Binding Protein Tomosyn" J Neurosci. 2016 Nov 2;36(44):11208-11222 PMID:27807164
Figure(s):	Fig. 1, 2
Annotation description:	In this paper, Cazares et al demonstrate a novel function of tomosyn-1 despite its effect on synaptic vesicle priming and ternary complex assembly. The authors monitor the dynamics of distinct synaptic vesicle pools via Vglut1-PhLuorin fluorescence measurements under Wt and tomosyn-1 KD conditions. They demonstrate by quantitative SV pool size measurement the redistribution of vesicles between total releasable pool, TRP (TRP= RRP (readily releasable pool) plus RP (recycling pool)) plus resting pool, ResP (Fig. 1,2). This activity-dependent mechanism of tomosyn-1 consists of clamping of SVs in the ResP and preventing them from transition in the TRP, a function which is regulated by Cdk5-mediated phosphorylation of tomosyn 1. Furthermore, the authors show that tomosyn operates by phosphorylation-dependent association with Rab3a-GTP and synapsin.
Evidence tracking, Biological System:	Cultured neurons
Evidence tracking, Protein Targeting:	RNAi / shRNA
Evidence tracking, Experiment Assay:	Wide-field fluorescence Whole-cell patch clamp
Annotator(s):	Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498)
Lab:	Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany
SynGO annotation ID:	1260
Dataset release (version):	20231201
View annotation as GO-CAM model: