Annotated protein:Glutamate receptor ionotropic, NMDA 2A (GluN2A) (Glutamate [NMDA] receptor subunit epsilon-1) (N-methyl D-aspartate receptor subtype 2A) (NMDAR2A) (NR2A). Gene symbol: GRIN2A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q00959
antibody wiki:
SynGO gene info:SynGO data @ GRIN2A
Ontology domain:Biological Process
SynGO term:ligand-gated ion channel activity involved in regulation of presynaptic membrane potential (GO:0099507)
Synapse type(s):cerebellum, glutamatergic
Annotated paper:Bidoret C, et al. "Presynaptic NR2A-containing NMDA receptors implement a high-pass filter synaptic plasticity rule" Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):14126-31 PMID:19666514
Figure(s):Fig. 2, 3, 4
Annotation description:Fig.2: "NR2A- but not NR2B-containing NMDAR are required for LTD induction" (rat cerebellum, Zn antagonist)
Fig.3: NR2A is exclusively located in the parallel fibers varicosities (presynapses)
Fig.4 Electrophysiological characterisation of NR2A overexposed in HEK cells using WC patch-clamp. Shows that the NR2A receptor regulates membrane potential (indrect via voltage-clamp)

Together these data show that the presynaptic NR2A containing receptor is active at the presynaptic plasma membrane and that here the firing properties of the presynaptic neuron are regulated via modulation of the presynaptic membrane potential to shape LTD induction presumably via Gutamate autoreception.

A model for the presynaptic action of the NMDARs is presented in Fig.5
Evidence tracking, Biological System:Intact tissue
Non-neuronal tissue
Evidence tracking, Protein Targeting:Over-expression
Antagonist / agonist
Evidence tracking, Experiment Assay:Whole-cell patch clamp
Annotator(s):Niels Cornelisse (ORCID:0000-0001-9425-2935)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:1352
Dataset release (version):20210225
View annotation as GO-CAM model:Gene Ontology