Annotated protein:Neuronal acetylcholine receptor subunit alpha-7. Gene symbol: CHRNA7. Taxonomy: Mus musculus (Mouse). Uniprot ID: P49582
antibody wiki:
SynGO gene info:SynGO data @ CHRNA7
Ontology domain:Cellular Component
SynGO term:integral component of presynaptic membrane (GO:0099056)
Synapse type(s):striatum
Annotated paper:Garcao P, et al. "Subsynaptic localization of nicotinic acetylcholine receptor subunits: a comparative study in the mouse and rat striatum" Neurosci Lett. 2014 Apr 30;566:106-10 PMID:24607281
Figure(s):fig 2B
Annotation description:Detection of nAchRs subunits in sub synaptic fractions of rodent striatal synaptosomes with Western Blot

22/11/2017 Pim
- The authors mention enrichment of AZ, but I allow annotation to the less specific term presynaptic membrane to reflect the dirty nature of the preparation.
- Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen.
Evidence tracking, Biological System:Intact tissue
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Western blot
Biochemical fractionation (generic)
Annotator(s):Niels Cornelisse (ORCID:0000-0001-9425-2935)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:1425
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology