| Annotated protein: | Rho guanine nucleotide exchange factor 9 (Collybistin) (Rac/Cdc42 guanine nucleotide exchange factor 9). Gene symbol: ARHGEF9. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9QX73 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ARHGEF9 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic specialization (GO:0099572) |
| Synapse type(s): | brain, GABAergic brain, glycinergic |
| Annotated paper: | Patrizi A, et al. "Selective localization of collybistin at a subset of inhibitory synapses in brain circuits" J Comp Neurol. 2012 Jan 1;520(1):130-41 PMID:21681748 |
| Figure(s): | Figs. 2 (rat), 3 (mouse), 4 (rat), 5 (rat) |
| Annotation description: | This publication assigns Collybistin immunoreactivity clearly to a subset of inhibitory synapses throughout the brain. Collybistin is an established interactor of Gephyrin, which was localized to the inhibitory postsynapse through immunogold as reviewed in 24552784. 10/2/2017 Pim Note: clusters of gephyrin, a postsynaptic protein, are used in this publication as a proxy for inh. synapses (see PMID:24552784). Fig.2: inh. (GABA) synapses in cerebellar ctx [rat] Fig.3: inh. (GABA) synapses in purkinje cells [mouse] Fig.4: various brain area's in brain [rat] Fig.5: colocalization with different GABA-A-R subunits in various brain area's, and with GlycRs in brainstem [rat] My final interpretation is that CB3 is present in the postsynaptic specialization (using gephyrin as a proxy) of GABA-ergic and Glycin-ergic synapses in the rodent brain (as also indicated by the Gundelfinger group). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Microscopy (generic) |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| Additional literature: | Identification of Collybistin as interactor of Gephyrin, a postsynaptic marker of inhibitory synapses. @ PMID:10607391 Review that supports gephyrin as a marker of postsynaptic specializations for inhibitory synapses in the CNS. @ PMID:24552784 |
| SynGO annotation ID: | 155 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |