Annotated protein:	Intersectin-1 (EH and SH3 domains protein 1). Gene symbol: ITSN1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9Z0R4
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SynGO gene info:	SynGO data @ ITSN1
Ontology domain:	Cellular Component
SynGO term:	presynaptic endocytic zone (GO:0098833)
Synapse type(s):	Calyx of Held
Annotated paper:	Sakaba T, et al. "Fast neurotransmitter release regulated by the endocytic scaffold intersectin" Proc Natl Acad Sci U S A. 2013 May 14;110(20):8266-71 PMID:23633571
Figure(s):	figure 1
Annotation description:	The authors used confocal (figure 1a) and superresolution stimulated emission depletion (STED) microscopy (figure 1-b-d) iin situ by preparing thin sections from the mature mouse calyx of Held. Immunostaining for the active zone protein bassoon revealed punctuate signals representing sites for rapid neurotransmitter release along the cup-like calyx terminal and at small conventional boutons. Intersectin was found at the calyx terminal. High-magnification STED imaging revealed a subset of intersectin puncta that overlapped with bassoon-containing AZs whereas a large fraction of intersectin puncta was also located peripheral to the bassoon hot spots in a non random manner. 17/11/2017 Pim - I have allowed annotation to the 'endocytic zone' because a concentration of the known endocytic protein intersectin 1 is demonstrated in close association to the active zone. Moreover the authors themselves claim as much: "Our data indicate a role for the endocytic scaffold intersectin in fast neurotransmitter release..."
Evidence tracking, Biological System:	Intact tissue
Evidence tracking, Protein Targeting:	Antibody (detection)
Evidence tracking, Experiment Assay:	Super resolution Confocal
Annotator(s):	Peter McPherson (ORCID:0000-0001-7806-5662)
Lab:	Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada
SynGO annotation ID:	1707
Dataset release (version):	20231201
View annotation as GO-CAM model: