| Annotated protein: | Disks large-associated protein 2 (DAP-2) (PSD-95/SAP90-binding protein 2) (SAP90/PSD-95-associated protein 2) (SAPAP2). Gene symbol: DLGAP2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8BJ42 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DLGAP2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic neurotransmitter receptor activity (GO:0098962) |
| Annotated paper: | Jiang-Xie LF, et al. "Autism-associated gene Dlgap2 mutant mice demonstrate exacerbated aggressive behaviors and orbitofrontal cortex deficits" Mol Autism. 2014 May 1;5:32 PMID:25071926 |
| Figure(s): | fig 4; fig 5; fig 6 a,b; fig 7b,c |
| Annotation description: | Figure 4 DLGAP2 regulates glutamate receptors localization at synapses. Literal "There was a clear downregulation of synaptic receptors GluR1 and NR1 in mutants, which may indicate that synaptic transmission is impaired (Figure 4A). Moreover, the levels of synaptic scaffold proteins Homer-1b/c and αCaMKII were also lower in the cortical synaptosomal fractions, which may imply a disruption to synaptic structures (Figure 4B)." Fig 5 DLGAP2 is essential for synapses formation Literal "Dlgap2-/-mice demonstrated a clear reduction of spine density (Figure 5B), indicating the pyramidal neurons received less excitable inputs in the OFC." Fig 6 DLGAP2 is essential for synaptic transmission Literal "We recorded AMPAR-mEPSCs in acute slices of the OFC. Clearly, our results with a lower peak mEPSC amplitude for Dlgap2-/-mice (Figure 6A,B) indicate that the postsynaptic responses of available synapses was weaker functionally. Fig 7b DLGAP2 is essential for synapses structure Literal "Strikingly, our results demonstrated a clear reduction in the length of the PSD in the OFC (Figure 7B).Additionally, the thickness of the PSD also had dramatic downregulation (Figure 7C). These results indicate the obvious deficits of postsynaptic ultra-structures in Dlgap2-/-mice. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Wide-field fluorescence Whole-cell patch clamp Biochemical fractionation (generic) |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 1780 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |