| Annotated protein: | Serine/threonine-protein kinase mTOR (EC 2.7.11.1) (FK506-binding protein 12-rapamycin complex-associated protein 1) (FKBP12-rapamycin complex-associated protein) (Mammalian target of rapamycin) (mTOR) (Mechanistic target of rapamycin) (Rapamycin target protein 1) (RAPT1). Gene symbol: MTOR. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P42346 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MTOR |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of translation at synapse, modulating synaptic transmission (GO:0099547) |
| Synapse type(s): | cerebral cortex |
| Annotated paper: | Takei N, et al. "Brain-derived neurotrophic factor induces mammalian target of rapamycin-dependent local activation of translation machinery and protein synthesis in neuronal dendrites" J Neurosci. 2004 Nov 3;24(44):9760-9 PMID:15525761 |
| Figure(s): | fig. 8 and fig. 9 |
| Annotation description: | mTOR siRNA transfection reduced mTOR (Fig. 8I) and phospho-p70S6K (Fig. 8J) in dendrites upon BDNF treatment in cortical cultures. Figure 8L shows the intensity of red fluorescence, which reflects phospho-S6K signal, in each dendrite that is measured. In Figure 9 the authors analysed phosph-4EBP levels in isolated dendrites. Even in isolated dendrites, BDNF increased and rapamycin decreased the levels of phosphorylated 4EBP (Fig. 9A-F), indicating the local activation of mTOR-dependent translation in dendrites. 14/11/2017 Pim - The BDNF-induced activation of protein translation is rapamycin-sensitive. Rapamycin is an antagonist of mTOR. - The phospho state of 4EBP1 is used as a readout of the efffect of mTOR on protein translation.\ - Literal: "BDNF increased protein synthesis in the synaptoneurosomes, a concentrated fraction of presynaptic and postsynaptic components prepared from rat brain" --> shows synaptic context |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Antagonist / agonist Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot |
| Annotator(s): | Alexandros Kanellopoulos (ORCID:0000-0002-2094-7491) Vittoria Mariano (ORCID:0000-0002-9848-0262) Achsel Tilmann (ORCID:0000-0002-1190-4481) Claudia Bagni (ORCID:0000-0002-4419-210X) |
| Lab: | Department of Fundamental Neurosciences, University of Lausanne, CH-1006 Lausanne, Switzerland; Dept Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy |
| SynGO annotation ID: | 1869 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |