Annotated protein:Neuronal cell adhesion molecule (Nr-CAM) (Neuronal surface protein Bravo) (mBravo) (NgCAM-related cell adhesion molecule) (Ng-CAM-related). Gene symbol: NRCAM. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q810U4
antibody wiki:
SynGO gene info:SynGO data @ NRCAM
Ontology domain:Cellular Component
SynGO term:integral component of postsynaptic membrane (GO:0099055)
Synapse type(s):cerebral cortex, glutamatergic
Annotated paper:Demyanenko GP, et al. "Neural cell adhesion molecule NrCAM regulates Semaphorin 3F-induced dendritic spine remodeling" J Neurosci. 2014 Aug 20;34(34):11274-87 PMID:25143608
Figure(s):Fig. 5D and E.
Annotation description:Fig. 5D and E, the double immunostaining showed colocalization of NrCAM (green) and Npn-2 (red) at numerous puncta (arrows) in confocal images of V1, layer 4 (P21) and dendritic spine puncta of pyramidal cells in V1, layer 4 (P21), labeled with EGFP by in utero electroporation and EGFP-positive, were labeled for both NrCAM and Npn2.

Literal:
"Double immunofluorescence staining showed colocalization of NrCAM and Npn-2 at numerous puncta in confocal images of mouse brain (P21) in sections of V1, layer 4, consistent with association studies in COS-7 cells (Fig. 5D). It was reported that Npn-2 colocalizes with PSD-95. In Figure 1C, NrCAM staining of puncta in V1, layer 4 (P21) overlapped with the postsynaptic marker PSD-95, while pyramidal cells labeled with EGFP by in utero electroporation showed punctate labeling at the tip of spines for both NrCAM and Npn2 (Fig. 5E).

9/11/2017 Pim
- Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen.
Evidence tracking, Biological System:Intact tissue
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Confocal
Annotator(s):Chiara Verpelli (ORCID:0000-0003-2949-9725)
Carlo Sala (ORCID:0000-0003-0662-9523)
Lab:CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy
SynGO annotation ID:1888
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology