| Annotated protein: | Fragile X messenger ribonucleoprotein 1 (Fragile X messenger ribonucleoprotein) (FMRP). Gene symbol: FMR1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q80WE1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ FMR1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic cytosol (GO:0099524) |
| Synapse type(s): | cerebral cortex, glutamatergic Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Weiler IJ, et al. "Fragile X mental retardation protein is translated near synapses in response to neurotransmitter activation" Proc Natl Acad Sci U S A. 1997 May 13;94(10):5395-400 PMID:9144248 |
| Figure(s): | Fig. 7 |
| Annotation description: | Fig. 7: Electron micrographs demonstrating FMRP localization in dendritic spines in cerebral cortex and hippocampus near PSD. 20/2/2017 Pim: -cytosol inferred from EM pictures and absence of membrane-localizing domains. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| Additional literature: | Figure 3: Fragile X mental-retardation protein (FMRP) colocalizes with target mRNA MAP1B in dendrites and at synapses. (a) Single-label immunofluorescence with single-label in situ hybridization in 19 DIV rat hippocampal neuron depicts FMRP (red) colocalization with MAP1B mRNA (green) in dendrites. Insert (1) is enlarged to show colocalization (yellow granules). (b) Insert (1) after Z-series and three-dimensional reconstruction and addition of immunofluorescent synapsin labeling (blue), to detect the presynaptic terminal. Insets a and b are enlarged at bottom, and show FMRP and MAP1B mRNA colocalization at synaptic sites (arrows). (c) Double-label immunofluorescence of FMRP and the dendritically localized mRNA-binding protein Staufen. Staufen is highly abundant in hippocampal dendrites, but it does not colocalize with FMRP in dendrites. @ PMID:16098134 |
| SynGO annotation ID: | 197 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |