| Annotated protein: | 5-hydroxytryptamine receptor 3A (5-HT3-A) (5-HT3A) (5-hydroxytryptamine receptor 3) (5-HT-3) (5-HT3R) (Serotonin receptor 3A) (Serotonin-gated ion channel receptor). Gene symbol: HTR3A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P35563 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ HTR3A |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | spinal cord |
| Annotated paper: | Maxwell DJ, et al. "Characterisation of axon terminals in the rat dorsal horn that are immunoreactive for serotonin 5-HT3A receptor subunits" Exp Brain Res. 2003 Mar;149(1):114-24 PMID:12592509 |
| Figure(s): | Fig.5 |
| Annotation description: | "There was a concentration of immunoreactivity in laminae I and II where numerous punctate structures and some cells were located (Fig. 4). Ultrastructural analysis confirmed that the punctate structures were synaptic boutons. In total, 33 boutons were examined with the electron microscope (Fig. 5); these invariably formed pronounced asymmetric synaptic junctions (Fig. 5A) with large and small dendritic profiles (Fig. 5A, D). Occasionally postsynaptic profiles contained vesicle-like structures and were tentatively identified as vesicle-containing dendrites (Fig. 5B)." - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2039 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |