| Annotated protein: | Disks large homolog 1 (Synapse-associated protein 97) (SAP-97) (SAP97). Gene symbol: DLG1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q62696 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DLG1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density, intracellular component (GO:0099092) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Valtschanoff JG, et al. "SAP97 concentrates at the postsynaptic density in cerebral cortex" Eur J Neurosci. 2000 Oct;12(10):3605-14 PMID:11029631 |
| Figure(s): | Figure 6; Figure 1 |
| Annotation description: | In layer II-III neurons of somatosenosry cortex, SAP97 localizes to the postsynaptic density of asymmetrical, excitatory synapses (Figure 6). SAP97 is also found in the dendritic cytoplasm. SAP97 antibody for immunogold labeling: A new antibody against SAP97 was generated by this group targeting the first 105 amino acids of SAP97, a region that does not contain sequence similarity to SAP102, PSD-95, PSD-93 or other known proteins. Antibody characterization and validation is shown is Figure 1. 29/01/2018 Pim - The more specific term 'anchored component of ... membrane' was NOT selected because: "the general consensus is that SAP-97 and SAP-102 are not lipid anchored but PSD-95 and PSD-93 are (See PMID 10779526). However, there is some evidence that some alternatively spliced isoforms of SAP-97 are palmitoylated (PMID 19357261)." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Hana Goldschmidt (ORCID:0000-0002-5676-366X) Richard Huganir (ORCID:0000-0001-9783-5183) |
| Lab: | Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA and Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA |
| Additional literature: | SAP97 localizes to the postsynaptic density of climbing fiber (presynaptic)/Purkinje cell (PC, postsynaptic) synapses in the rat cerebellar cortex. Localization is developmentally regulated and SAP97 (along with GluA1 and 4.9N) is not present at these synapses after the second postnatal week. In adult rats, SAP97 is found in the cytoplasm and intracellular organelles (ER) of Bergmann glia, whose processes wrap around the PC. Fig 6, Fig. 7A-C @ PMID:17335044 SAP97 binds to GluA1-AMPA receptors while they are still in the endoplasmic reticulum (ER) and that most SAP97 is released upon receptor arrival at the synapse. Hippocampal CA1 synapses; Figure 5: SAP97 found in cytoplasm associated with ER cisternae and in PSD. Synaptic labeling was low (28% synapses were labeled) Sprague Dawley rats @ PMID:11567040 |
| SynGO annotation ID: | 2049 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |