Annotated protein:Unconventional myosin-Va (Dilute myosin heavy chain, non-muscle). Gene symbol: MYO5A. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q99104
antibody wiki:
SynGO gene info:SynGO data @ MYO5A
Ontology domain:Biological Process
SynGO term:establishment of endoplasmic reticulum localization to postsynapse (GO:0099089)
Synapse type(s):cerebellum, glutamatergic
Annotated paper:Wagner W, et al. "Myosin-Va transports the endoplasmic reticulum into the dendritic spines of Purkinje neurons" Nat Cell Biol. 2011 Jan;13(1):40-8 PMID:21151132
Figure(s):Fig.1-4
Annotation description:Fig.1: "Absence of the delayed mGluR1-dependent Ca2+ transient in dl20J/dl20J PN spines and hypothetical models for how myosin-Va might function to localize the ER Ca2+ store to spines"
Fig.2: "The translocation of ER into spines is disrupted in dl20J/dl20J PNs"
Fig.3: "mGFP-myosin-Va expressed in dl20J/dl20J PNs rescues ER targeting and accumulates at the tip of the spine ER"
Fig.4: "Myosin-Va is present at the leading tip of the ER tubule as the organelle translocates into a spine"
Evidence tracking, Biological System:Intact tissue
Cultured neurons
Evidence tracking, Protein Targeting:Genetic transformation (eg; knockout, knockin, mutations)
Over-expression
Evidence tracking, Experiment Assay:Confocal
Two-photon microscopy
Annotator(s):Harold MacGillavry (ORCID:0000-0002-6153-3586)
Casper Hoogenraad (ORCID:0000-0002-2666-0758)
Lab:Department of Biology, Faculty of Science, Utrecht University, 3584 CH Utrecht, The Netherlands
SynGO annotation ID:2143
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology