| Annotated protein: | Potassium voltage-gated channel subfamily D member 2 (Voltage-gated potassium channel subunit Kv4.2). Gene symbol: KCND2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9Z0V2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KCND2 |
| Ontology domain: | Biological Process |
| SynGO term: | voltage-gated ion channel activity involved in regulation of postsynaptic membrane potential (GO:1905030) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Kim J, et al. "Regulation of dendritic excitability by activity-dependent trafficking of the A-type K+ channel subunit Kv4.2 in hippocampal neurons" Neuron. 2007 Jun 21;54(6):933-47 PMID:17582333 |
| Figure(s): | Fig.6 |
| Annotation description: | Shown is an activity-dependent redistribution of Kv4.2 out of dendritic spines in cultured hippocampal neurons. This redistribution requires NMDAR activation and Ca2+ influx and occurs through clathrin-mediated endocytosis. Glycine-induced long-term potentiation (chem-LTP) resulted in concurrent synaptic GluR1 insertion and Kv4.2 internalization. Whole-cell recordings supported these results, showing that endogenous A-type, but not sustained, K+ currents are reduced after chem-LTP induction. Expression of a Kv4.2 dominant-negative mutant was found to increase mEPSC amplitudes and stimuli that induce Kv4.2 internalization resulted in enhanced synaptic currents. Finally, in hippocampal organotypic slice cultures we show that synaptically induced LTP is reduced by hyperpolarization, suggesting that Kv4.2 internalization contributes to LTP. Activity-dependent regulation of Kv4.2 surface expression thus presents a synapse-specific molecular mechanism for the fine-tuning of dendritic signals. Fig.6: "Kv4.2 internalization affects dendritic integration of synaptic input" |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Niels Cornelisse (ORCID:0000-0001-9425-2935) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | KCND2 / Kv4.2 has a molecular function of a voltage gated potassium channel as shown by expression in HEK cells. @ PMID:15208026 |
| SynGO annotation ID: | 2159 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |