| Annotated protein: | Glutamate receptor-interacting protein 1 (GRIP-1) (AMPA receptor-interacting protein GRIP1). Gene symbol: GRIP1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P97879 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GRIP1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | neocortex, glutamatergic hippocampus, glutamatergic |
| Annotated paper: | Dong H, et al. "Characterization of the glutamate receptor-interacting proteins GRIP1 and GRIP2" J Neurosci. 1999 Aug 15;19(16):6930-41 PMID:10436050 |
| Figure(s): | Figure 2, 7 |
| Annotation description: | Fig.2: "Distribution and subcellular fractionation of GRIP1 and GRIP2 protein in brain" - Literal: "Treatment of synaptic plasma membrane fractions with various detergents to generate PSD fractions demonstrated that GRIP1 and GRIP2 were enriched in the PSD (Fig.2 e)" Fig.4: "In vivo and in vitro interaction of full-length GRIP1 and AMPA receptors" Literal: " These results demonstrate that GRIP1 and GRIP2 can associate with AMPA receptorsin situ in transfected cells and that GRIP1 is associated with AMPA receptors in vivo in brain" Fig.7: "Immunogold electron microscopy localization of GRIP1 protein in CNS" - Literal: "Most frequently, the gold clusters appeared in dendrites (Fig. 7 b, arrows) and were typically associated with vesicles, sometimes clearly outlining a vesicular profile. These gold-labeled vesicles were often very close to microtubule filaments. Immunogold label was also found in postsynaptic spines, frequently near the PSD region (Fig. 7 c-e, arrows), and was occasionally found within the PSD (Fig. 7 c,arrows)." I conclude from this data that when inside a synaptic region (synaptosomal preparation), Grip1 is preferentially localized in the PSD. However, the localization to undefined dendritic compartments may represent additional synapse-associated organelles involved in NTR trafficking. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Western blot Biochemical fractionation (generic) |
| Annotator(s): | Harold MacGillavry (ORCID:0000-0002-6153-3586) Casper Hoogenraad (ORCID:0000-0002-2666-0758) |
| Lab: | Department of Biology, Faculty of Science, Utrecht University, 3584 CH Utrecht, The Netherlands |
| SynGO annotation ID: | 2231 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |