| Annotated protein: | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12) (Peptidyl-cysteine S-nitrosylase GAPDH) (EC 2.6.99.-). Gene symbol: GAPDH. Taxonomy: Bos taurus (Bovine). Uniprot ID: P10096 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GAPDH |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synaptic vesicle neurotransmitter loading (GO:0099162) |
| Synapse type(s): | cerebrum |
| Annotated paper: | Ikemoto A, et al. "Glycolysis and glutamate accumulation into synaptic vesicles. Role of glyceraldehyde phosphate dehydrogenase and 3-phosphoglycerate kinase" J Biol Chem. 2003 Feb 21;278(8):5929-40 PMID:12488440 |
| Figure(s): | Figs. 3 |
| Annotation description: | Results section: "Synaptic Vesicles Are Capable of Accumulating Glu via Activation of Endogenous GAPDH/3-PGK" - Literal: "Synaptic vesicles were incubated for various periods with [3H]Glu in the presence or absence of 2 mM GAP, NAD, and Pi and 0.1 mM ADP. Vesicular Glu uptake was dependent on the presence of the GAPDH/3-PGK substrate mixture throughout the incubation period(s) tested and was approximately linear up to 6 min under these conditions (Fig. 3A). When any one of the components was omitted from the incubation mixture, vesicular Glu uptake was not supported (Fig. 3B)." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antagonist / agonist Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) Transmembrane transport assay |
| Annotator(s): | Rita Reig-Viader (ORCID:0000-0002-6893-6177) Àlex Bayés (ORCID:0000-0002-5265-6306) |
| Lab: | Molecular Physiology of the Synapse Laboratory, Biomedical Research Institute Sant Pau, 08025 Barcelona, Spain and and Universitat Autnoma de Cerdanyola del Valls, Spain Barcelona, 08193 Bellaterra |
| SynGO annotation ID: | 2398 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |