| Annotated protein: | Tuberin (Tuberous sclerosis 2 protein homolog). Gene symbol: TSC2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P49816 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ TSC2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse organization (GO:0099175) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Yasuda S, et al. "Activation of Rheb, but not of mTORC1, impairs spine synapse morphogenesis in tuberous sclerosis complex" Sci Rep. 2014 Jun 3;4:5155 PMID:24889507 |
| Figure(s): | Figure 1 |
| Annotation description: | FIGURE 1 Tsc2 mutation suppresses dendritic spine synapse formation. (a) Images of dendrites from wild-type (top) and Tsc2+/- (bottom) pyramidal neurons in hippocampal culture. Neurons were transfected with EGFP and immunostained with anti-vGlut1 antibody. Scale bar, 10μm. (b, c) Cumulative probability plots of protrusion width and length shown in (a). Tsc2+/- neurons showed a decrease in protrusion width (D = 0.2515, P < 0.001) and an increase in protrusion length (D = 0.4127, P < 0.001) compared with WT neurons (Kolmogorov-Smirnov test). (d) Densities of vGlut1-positive spine synapses in WT and Tsc2+/- neurons (***P < 0.001, unpaired, two-tailed t-test). (e) Percentage of spine synapses in WT and Tsc2+/- neurons (***P < 0.001, unpaired, two-tailed t-test). For each experiment (a-e), a total of 1,060/15/5 (WT) and 1,007/15/5 (Tsc2+/-) protrusions/neurons/experiments were analysed, respectively. (f) Representative DiI-labelled dendrites of hippocampal CA1 neurons from WT and Tsc2+/- rats. Scale bar, 10μm. (g, h) Cumulative probability plots of the protrusion width (g) and length (h) shown in (f). Tsc2+/- neurons showed a decrease in the protrusion width (D = 0.2950, P < 0.001) and an increase in the protrusion length (D = 0.2256, P < 0.001) compared with WT neurons (n = 1,001/12/6 (WT) and 1,034/12/6 (Tsc2+/-) protrusions/neurons/experiments (Kolmogorov-Smirnov test). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Alexandros Kanellopoulos (ORCID:0000-0002-2094-7491) Vittoria Mariano (ORCID:0000-0002-9848-0262) Achsel Tilmann (ORCID:0000-0002-1190-4481) Claudia Bagni (ORCID:0000-0002-4419-210X) |
| Lab: | Department of Fundamental Neurosciences, University of Lausanne, CH-1006 Lausanne, Switzerland; Dept Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy |
| SynGO annotation ID: | 2415 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |