| Annotated protein: | non-specific serine/threonine protein kinase (EC 2.7.11.1). Gene symbol: PAK. Taxonomy: Drosophila melanogaster (Fruit fly). Uniprot ID: B7Z0W0 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PAK1 SynGO data @ PAK2 SynGO data @ PAK3 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of neurotransmitter receptor localization to postsynaptic specialization membrane (GO:0098696) |
| Synapse type(s): | Neuro-muscular junction |
| Annotated paper: | Albin SD, et al. "Coordinating structural and functional synapse development: postsynaptic p21-activated kinase independently specifies glutamate receptor abundance and postsynaptic morphology" J Neurosci. 2004 Aug 4;24(31):6871-9 PMID:15295021 |
| Figure(s): | Figure 3, 7 |
| Annotation description: | Figure 3. Decreased quantal size and normal homeostatic compensation at Pak mutant NMJ. A, Quantification of quantal size in control (filled bar) and experimental genotypes (open bars). Wild-type and heterozygous controls [Df(3R)Win11/+ and Pak6/+; GluRIIASP16/+] have quantal sizes equal to 1.1 ± 0.1 mV (n = 6), 0.98 ± 0.7 mV (n = 6), and 0.96 ± 0.83 mV (n = 5), respectively. Experimental genotypes all showed significant decreases in quantal size: Pak6/Df(3R)Win11, 0.73 ± 0.07 mV, n = 6; GluRIIASP16, 0.51 ± 0.01 mV, n = 18; GluRIIASP16; Pak6/+, 0.46 ± 0.02 mV, n = 5; GluRIIASP16; Pak3/Pak6, 0.4 ± 0.01, n = 7. There is also a small, yet statistically significant, difference between GluRIIASP16 and GluRIIASP16; Pak3/Pak6. B, Quantification of quantal content in control (filled bars) and experimental genotypes (open bars). There is no difference in quantal content comparing the experimental genotype Pak6/Df(3R) Win11 with wildtype or experimental controls. Values are as follows: wildtype, 34.5 ± 1.5, n = 6; Df(3R)Win11/+, 32.1 ± 2.8, n = 6; Pak6/+; GluRIIASP16/+, 39.3 ± 5.2, n = 5; Pak6/Df(3R)Win11, 40.7 ± 3.7, n = 6. The experimental genotypes GluRIIASP16, GluRIIASP16; Pak6/+, and GluRIIASP16; Pak3/Pak6 all showed significant increases in quantal content compared with wild type and genetic controls, indicating that homeostatic compensation has occurred. Values are as follows: GluRIIASP16, 58 ± 3.7 mV, n = 15; GluRIIASP16; Pak6/+, 48.8 ± 5.2 mV, n = 5; and GluRIIASP16; Pak3/Pak6, 63.6 ± 8.7, n = 6. C, Representative traces of evoked potentials (left; each trace represents the average of 10 individual traces) and spontaneous miniature potentials (right) from control [Df(3R)Win11/+] and Pak mutant NMJ [Pak6/Df(3R)Win11]. The traces show the reduction in quantal size in Pak mutant animals and the wild-type EPSP amplitude indicative of effective synaptic homeostasis. Calibration: 10 mV, 50 msec (for evoked release); 1 mV, 250 msec (for spontaneous traces). Significance is denoted as *p < 0.05; **p < 0.002; ***p < 0.00002. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence Electrophysiology (generic) |
| Annotator(s): | Alexandros Kanellopoulos (ORCID:0000-0002-2094-7491) Vittoria Mariano (ORCID:0000-0002-9848-0262) Achsel Tilmann (ORCID:0000-0002-1190-4481) Claudia Bagni (ORCID:0000-0002-4419-210X) |
| Lab: | Department of Fundamental Neurosciences, University of Lausanne, CH-1006 Lausanne, Switzerland; Dept Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy |
| SynGO annotation ID: | 2432 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |