| Annotated protein: | Neuroligin-2. Gene symbol: NLGN2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q62888 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NLGN2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic specialization membrane (GO:0099060) |
| Synapse type(s): | cerebellum, GABAergic hippocampus, GABAergic |
| Annotated paper: | Varoqueaux F, et al. "Neuroligin 2 is exclusively localized to inhibitory synapses" Eur J Cell Biol. 2004 Sep;83(9):449-56 PMID:15540461 |
| Figure(s): | Fig.4, 5, 6, 8 |
| Annotation description: | Fig.4: "Ultrastructural localization of NL2 by postembedding immunogold labeling with an anti-NL2 antibody" - "At the ultrastructural level, NL2 immunogold labeling was associated with the plasma membrane at symmetric synapses (Fig. 4G) and to a lesser extent within dendrites, often onto tubular structures close to synapses" Fig.5: "Synaptic localization of NL2 in the cerebellar cortex" - "NL2-positive structures were exactly facing synaptophysin-labeled presynaptic compartments (Fig. 5A)." - "In regions known to receive GABAergic innervation, such as Purkinje cell bodies, NL2 puncta were not colocalized with vGlut1 (Fig. 5B), whereas vGAT labeling was found always directly adjacent to NL2 (Fig. 5C). Likewise, the abundance, distribution, shape, and size of NL2-positive puncta in the glomeruli closely correlated with the vGAT staining (Fig. 5C)." Fig.6: "Distribution of NL2 at inhibitory postsynapses in mature cultured hippocampal neurons" Fig.8: "Appearance of NL2 clusters and inhibitory synaptic markers during the development of hippocampal neurons in culture." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2755 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |