| Annotated protein: | Neuroligin-3 (Gliotactin homolog). Gene symbol: NLGN3. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q62889 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NLGN3 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic specialization membrane (GO:0099060) |
| Synapse type(s): | hippocampus, GABAergic hippocampus, glutamatergic |
| Annotated paper: | Budreck EC, et al. "Neuroligin-3 is a neuronal adhesion protein at GABAergic and glutamatergic synapses" Eur J Neurosci. 2007 Oct;26(7):1738-48 PMID:17897391 |
| Figure(s): | Fig.2, 3, 4, 6 |
| Annotation description: | Fig.2: "NL3 in rodent brain is enriched in synaptic membrane fractions." - "A significant fraction of NL3 was recovered in the Triton X-100-insoluble postsynaptic density fraction containing PSD95 and NMDA (NR1) and AMPA (GluR2/3) glutamate receptors (Fig. 2B). NL3 was also detected in other subcellular fractions, which might reflect the existence of nonsynaptic and intracellular pools of NL3 (see below)." Fig.3: "NL3 is highly expressed in hippocampal neurons." - "We observed punctate staining along neuronal processes costained with the dendritic marker MAP2 (data not shown). Double-labelling with a presynaptic vesicle marker, synaptobrevin/VAMP2, indicated that a significant proportion of the NL3-positive structures are apposed to presynaptic terminals (Fig. 3A). " Fig.4: "NL3 is localized at both glutamatergic and GABAergic synapses in hippocampal neurons. " Fig.6: " NL3 is colocalized with NL2 at single synapses in vitro and is associated in separate complexes with NL1 and NL2 in vivo" Note: the localization data do not strictly point to localization in the postsynapse. PSD enrichment might be via tight transsynaptic protein interaction. This localization is assumed, however, based on the postsynaptic localization of other neuroligins paralogs and on the functional data (Fig.5) that shows recruitment of postsynaptic components following clustering of NLGN3; neurologin-3. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2767 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |