| Annotated protein: | Ephrin type-B receptor 2 (EC 2.7.10.1) (Neural kinase) (Nuk receptor tyrosine kinase) (Tyrosine-protein kinase receptor EPH-3) (Tyrosine-protein kinase receptor SEK-3) [Cleaved into: EphB2/CTF1; EphB2/CTF2]. Gene symbol: EPHB2. Taxonomy: Mus musculus (Mouse). Uniprot ID: P54763 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EPHB2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synapse assembly (GO:0051963) |
| Synapse type(s): | neocortex, glutamatergic |
| Annotated paper: | Kayser MS, et al. "Intracellular and trans-synaptic regulation of glutamatergic synaptogenesis by EphB receptors" J Neurosci. 2006 Nov 22;26(47):12152-64 PMID:17122040 |
| Figure(s): | fig 1, fig 3, fig. 4, fig 5, fig 6, fig 7 |
| Annotation description: | Fig 1. Cultured rat neurons at DIV 7 that overexpress a kinase-dead mutant of flag-tagged EphB2 (fEphB2_KD) form significantly less spines compared with WT EphB2 (fEphB2) and EphB2 lacking its PDZ domain (fEphB2deltaPDZ). Fig. 3/4 fEphB2 expressed in heterologous cells co-cultured with neurons triggers in contacting axons clustering of presynaptic vesicles (SV2 staining) in synapses that are functional (FM4-64 uptake). Fig 5. Knockdown of EphB2 in cultured cortical neurons results in decreased presynaptic puncta, spine number, and mEPSCs. Fig 6. Synapse density as measured by immunohistochemistry in sections of cortex from early postnatal mice lacking EphB1, EphB2, and EphB3 is reduced. Fig 7. EphB2 expression in cultured cortical sections of EphB1, 2, 3 KO mice is sufficient to rescue decreased PSD95 puncta on protrusions. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) RNAi / shRNA Over-expression Antagonist / agonist Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Electrophysiology (generic) |
| Annotator(s): | Tony Cijsouw (ORCID:0000-0003-0912-1514) Thomas Biederer (ORCID:0000-0002-3670-7863) |
| Lab: | Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA |
| SynGO annotation ID: | 278 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |