| Annotated protein: | Rap guanine nucleotide exchange factor 4 (Exchange factor directly activated by cAMP 2) (Exchange protein directly activated by cAMP 2) (EPAC 2) (cAMP-dependent Rap1 guanine-nucleotide exchange factor) (cAMP-regulated guanine nucleotide exchange factor II) (cAMP-GEFII). Gene symbol: RAPGEF4. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9EQZ6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RAPGEF4 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synaptic vesicle cycle (GO:0098693) |
| Synapse type(s): | Mossy fiber synapse (DG->CA3) |
| Annotated paper: | Fernandes HB, et al. "Epac2 Mediates cAMP-Dependent Potentiation of Neurotransmission in the Hippocampus" J Neurosci. 2015 Apr 22;35(16):6544-53 PMID:25904804 |
| Figure(s): | Fig.2, 3, 7 |
| Annotation description: | Fig.2: "cAMP-mediated potentiation of transmitter release is impaired in Epac2-/- mice" Fig.3: "MF-LTP is impaired in Epac2-/- mice. A, Time course of MF-LTP in Epac2+/+ mice" Fig.7: "Functional estimates of the total number of synaptic vesicles available for release in Epac2+/+ and Epac2-/- MF boutons" - " these data indicate that N, the number of readily releasable vesicles, is significantly reduced in Epac2-/- mice... This study proposes a novel mechanism for enhancement of transmitter release at MF synapses involving a noncanonical cAMP target and uncovers a novel role for Epac2 as a synapse-specific regulator of the RRP of vesicles." Note: LTP in the MF->CA3 synapse is known to be mediated by a presynaptic mechanism. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2852 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |