| Annotated protein: | Homer protein homolog 1 (PSD-Zip45) (VASP/Ena-related gene up-regulated during seizure and LTP 1) (Vesl-1). Gene symbol: HOMER1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9Z214 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ HOMER1 |
| Ontology domain: | Biological Process |
| SynGO term: | structural constituent of postsynapse (GO:0099186) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Lu J, et al. "Postsynaptic positioning of endocytic zones and AMPA receptor cycling by physical coupling of dynamin-3 to Homer" Neuron. 2007 Sep 20;55(6):874-89 PMID:17880892 |
| Figure(s): | Fig.4 |
| Annotation description: | Fig.4: "A Dyn3-Homer-Shank Protein Complex Couples the EZ and the PSD" - "To further test this model, we took advantage of Homer1a, a naturally occurring activity-induced isoform of Homer that lacks its C-terminal coiled-coil domain (Sala et al., 2003). Expression of Homer1a caused a marked increase in the fraction of synapses lacking a postsynaptic EZ (percent EZ-negative synapses: control, 8.4 ± 1.8%, n = 134; Homer1a, 38.7 ± 2.6%, n = 37; Figure 4G), that was quantitatively similar to that observed upon expression of Dyn3-PRD or Dyn3-P800L (Figure 4F). Next, we tested whether the dynamin-3/Homer-dependent localization of the EZ required Homer binding to Shank. Indeed, expression of either a C-terminal fragment of Shank that binds Homer (Shank C-term) or a full-length Shank mutant lacking four amino acids required for Homer binding (ShankΔPLEF) (Sala et al., 2001; Sala et al., 2003) likewise resulted in a significant loss of PSD-associated clathrin puncta (percent EZ-negative synapses: Shank C-term, 43.7 ± 3.6%, n = 12; ShankΔPLEF, 36.8 ± 6.3%, n = 21; p < 0.001 relative to control, t-test; Figure 4G). Thus, a linear sequence of protein-protein interactions involving Shank, Homer, and dynamin-3 is required to localize EZ to the lateral margins of the PSD." 20/01/2021 Dnyanada: Q9Z214 (HOMER1_RAT) was chosen on the basis of the biological system in which the experiment was conducted as the species of the overexpressed HOMER1b/c construct could not be retrieved. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | See also. @ PMID:24966368 |
| SynGO annotation ID: | 2921 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |