Annotated protein:Cadherin-9 (T1-cadherin). Gene symbol: CDH9. Taxonomy: Mus musculus (Mouse). Uniprot ID: P70407
antibody wiki:
SynGO gene info:SynGO data @ CDH9
Ontology domain:Biological Process
SynGO term:synapse adhesion between pre- and post-synapse (GO:0099560)
Synapse type(s):hippocampus, glutamatergic
Annotated paper:Basu R, et al. "Heterophilic Type II Cadherins Are Required for High-Magnitude Synaptic Potentiation in the Hippocampus" Neuron. 2017 Sep 27;96(1):160-176.e8 PMID:28957665
Figure(s):Fig.4
Annotation description:Fig.4: "Cadherin-9 Mediates Trans-cellular Adhesion via Cadherins-6 and -10"
Cell aggregation assay:
-"To determine whether cadherin-9 can bind in trans to other cadherins expressed in the hippocampus, we conducted cell aggregation assays (Takeichi and Nakagawa, 2001)."..."we identified four heterophilic cadherin pairs; cadherins-6/9, -9/10, -10/6, and -8/11 (Figures S4C and S4D)."
- "We then expressed Cdh9-smFPFLAG in one set of CHO cells to simulate CA3 neurons and plated them with a second set of CHO cells expressing Cdh6-smFPHA, Cdh10-smFPMYC, or Cdh6-smFPHA and Cdh10-smFPMYC (Figure 4C) to simulate CA1 neurons. Immunostaining shows that cadherins localize at the interaction interfaces in all combinations tested (Figures 4C and S4E)."
- "All cadherins tested were enriched in the synaptosome fraction relative to lysates by P14 (Figures 4E). "
- "We co-cultured neurons expressing Cdh9-smFPFLAG with neurons expressing Cdh6-smFPHA, Cdh10-smFPMYC, or both Cdh6-smFPHA and Cdh10-smFPMYC and immunostained for epitope tags and the synaptic markers vGLUT1 and PSD95. We observed co-localization of every pairwise combination of cadherins at synapses (Figures 4G-4I). "..."all three cadherins simultaneously co-localize at synaptic sites marked by juxtaposed vGLUT and PSD95 puncta (Figure 4J)."

In Fig.2 and 5 the in situ consequence of the adhesion activity for the Cadherin-6/9/10 is shown via effects on spine morphology and LTP (not annotated).
Evidence tracking, Biological System:Cultured neurons
Non-neuronal tissue
Evidence tracking, Protein Targeting:Over-expression
Evidence tracking, Experiment Assay:Confocal
Protein-protein interaction (generic)
Annotator(s):Pim van Nierop (ORCID:0000-0003-0593-3443)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:2955
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology