| Annotated protein: | Cullin-3 (CUL-3). Gene symbol: CUL3. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9JLV5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CUL3 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation protein catabolic process at postsynapse (GO:0140252) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Salinas GD, et al. "Actinfilin is a Cul3 substrate adaptor, linking GluR6 kainate receptor subunits to the ubiquitin-proteasome pathway" J Biol Chem. 2006 Dec 29;281(52):40164-73 PMID:17062563 |
| Figure(s): | Fig.6, 7 |
| Annotation description: | Title: "Actinfilin Is a Cul3 Substrate Adaptor, Linking GluR6 Kainate Receptor Subunits to the Ubiquitin-Proteasome Pathway" Note: Cul3; Cullin-3 is a "scaffold for the Cul3-based E3 ubiquitin-ligase complex (30-32)." Fig.6: "Cul3 regulates GluR6 expression in vivo" Fig.7: "Actinfilin and Cul3 negatively regulate surface expression of GluR6" - "We found that decreasing actinfilin levels via RNAi significantly increased surface GluR6 expression in dendritic spines, as assessed morphologically and by increased co-localization with PSD-95 (Fig. 7, A-D and H) and synaptophysin (Fig. 7H). Synaptic GluR6 more than doubled (Fig. 7H), and the size of the GluR6-containing puncta increased 1.8-fold (mean punctal area ± S.E.: control neurons, 0.12 ± 0.01 μm2; RNAi-AF expressing neurons, 0.22 ± 0.01 μm2). " |
| Evidence tracking, Biological System: | Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3040 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |