| Annotated protein: | Kaptin (Actin binding protein) (Predicted), isoform CRA_a (Kptn protein). Gene symbol: KPTN. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: B2RYG4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KPTN |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic actin cytoskeleton (GO:0098871) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Baple EL, et al. "Mutations in KPTN cause macrocephaly, neurodevelopmental delay, and seizures" Am J Hum Genet. 2014 Jan 2;94(1):87-94 PMID:24239382 |
| Figure(s): | Fig.2 |
| Annotation description: | Fig.2: " - "At later stages of development, when neurons established synapses (DIV14), wild-type Flag-kaptin again colocalized with F-actin-rich sites. Along dendrites, these sites appeared mainly to represent F-actin-rich postsynapses, given that almost all kaptin-enriched puncta were contacted by presynaptic structures containing bassoon (a marker for presynaptic active zones) (Figure 2C)." - "The vast majority of anti-kaptin-immunolabeled puncta were not only enriched with F-actin but were additionally immunopositive for Shank2, a postsynaptic scaffold protein interacting with F-actin binding proteins;12,13 Figure 2D). " Note: I do not think that this data is able to demonstrate localization to the actin cytoskeleton as claimed by the authors. Co-localization observed with confocal microscopy does not mean physical association. However, Kaptin is known to bind F-actin (see Additional literature) which allows for inference to the term 'postsynaptic actin cytoskeleton (GO:0098871)'. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Kaptin binds F-actin. @ PMID:10099934 |
| SynGO annotation ID: | 3056 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |