| Annotated protein: | Peripheral-type benzodiazepine receptor-associated protein 1 (PRAX-1) (Peripheral benzodiazepine receptor-interacting protein) (PBR-IP) (RIMS-binding protein 1) (RIM-BP1) (TSPO-associated protein 1). Gene symbol: TSPOAP1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q7TNF8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ TSPOAP1 |
| Ontology domain: | Biological Process |
| SynGO term: | voltage-gated calcium channel activity involved in regulation of presynaptic cytosolic calcium levels (GO:0099626) |
| Synapse type(s): | hippocampus, glutamatergic brainstem, glutamatergic Calyx of Held |
| Annotated paper: | Acuna C, et al. "RIM-BPs Mediate Tight Coupling of Action Potentials to Ca(2+)-Triggered Neurotransmitter Release" Neuron. 2015 Sep 23;87(6):1234-1247 PMID:26402606 |
| Figure(s): | Figure 2, 3, 4, 7 |
| Annotation description: | This publication provides some evidence that RIMBP1 (a gene product of BZRAP1) is involved in tethering calcium channels to release sites. RIMBP1/2 double knockout reduces synaptic transmission in cultured neurons (figure 2) and the calyx of held (figure 3). The remaining synaptic transmission displayed slowed kinetics (figure 4) and was sensitive to the slow calcium chelator EGTA (figure 7). This suggests an increased distance between calcium channels and the calcium sensor for vesicle fusion. This role of RIMBP1 in this process is inferred from sequence homology with RIMBP2 and the prominent brain expression of both genes. The RIMBP1 single knockout has not been studied. It should also be noted that Grauel et.al. (2016) did not detect a difference between the RIMBP2 KO and RIMBP1/2 double knockout. The exact contribution of of RIMBP1 is thus unknown. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| Additional literature: | In this publication, there is no added effect of RIMBP1 knockout on top of RIMBP2 knockout. @ PMID:27671655 Liu et.al. demonstrated a similar phenotype for RIMBP knockout in drosophila. @ PMID:22174254 |
| SynGO annotation ID: | 308 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |