| Annotated protein: | RIM-binding protein, isoform F. Gene symbol: RBP. Taxonomy: Drosophila melanogaster (Fruit fly). Uniprot ID: A0A0B4JDC9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ TSPOAP1 SynGO data @ RIMBP2 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic active zone cytoplasmic component (GO:0098831) |
| Synapse type(s): | Neuro-muscular junction |
| Annotated paper: | Liu KS, et al. "RIM-binding protein, a central part of the active zone, is essential for neurotransmitter release" Science. 2011 Dec 16;334(6062):1565-9 PMID:22174254 |
| Figure(s): | Figure 1, 2 |
| Annotation description: | Using two specific antibodies and STED microscopy, it is shown that RIMBP localizes to active zones in drosophila NMJ. RIMBP tightly colocalizes with Bruchpilot, a well established marker for the active zone (Figure 1). Figure 2 provides two knockout controls for the c-terminal antibody. Due to the high sequence homology between fly RIMBP and mammalian RIMBP1 & 2, and based on the fact that mouse RIMBP2 is localized at the active zone (Grauel et.al. 2016), it is assumed that mouse RIMBP1 is also present at the active zone. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Super resolution |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| Additional literature: | Grauel et al. demonstrates that RIMBP2, a close homolog of RIMBP1, is localized to the presynaptic active zone in mice. @ PMID:27671655 |
| SynGO annotation ID: | 309 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |