| Annotated protein: | Connector enhancer of kinase suppressor of ras 2 (Connector enhancer of KSR 2) (CNK homolog protein 2) (CNK2) (Membrane-associated guanylate kinase-interacting protein) (Maguin). Gene symbol: CNKSR2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9Z1T4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CNKSR2 |
| Ontology domain: | Cellular Component |
| SynGO term: | extrinsic component of postsynaptic density membrane (GO:0099147) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Yao I, et al. "MAGUIN, a novel neuronal membrane-associated guanylate kinase-interacting protein" J Biol Chem. 1999 Apr 23;274(17):11889-96 PMID:10207009 |
| Figure(s): | Fig.3, 5, 7, 8, 10 |
| Annotation description: | Fig.3: "Interaction of MAGUIN-1 with S-SCAM." - "...interaction of S-SCAM with the C-terminal region of MAGUIN-1. The extract of COS cells expressing S-SCAM was incubated with either GST-MAGUIN-12 (the C-terminal construct of MAGUIN-1) ..." Fig.5: "Interaction of MAGUIN-1 with PSD-95/SAP90" - "...coimmunoprecipitation of MAGUIN-1 with PSD-95/SAP90. The Triton X-100 extract of the rat crude synaptosomal fraction ..." Fig.7: "Subcellular localization of MAGUINs" - "...In the subcellular fractionation of rat brain, MAGUINs were detected mainly in the synaptic plasma membrane (SPM) and PSD fractions (Fig.7 A)..." - "In rat hippocampal neurons, MAGUINs were distributed in the cell body and the neurites and colocalized with NMDAR1 (Fig. 7 B)." Note: NMDARs are predominantly located in the PSD. Fig.8: "Membrane association of MAGUINs in CHO cells." - "The PH domain is known to interact with the phospholipid membrane...these findings suggest that MAGUIN-1 associates with the plasma membrane through the region containing the PH domain and the C-terminal stretch of the PH domain..." Fig.10: "Recruitment of PSD-95/SAP90 and S-SCAM into the Triton X-100-insoluble fraction by MAGUIN-1" - "A and B). MAGUIN-1 and -2 were distributed in the Triton X-100-insoluble fraction (Fig. 9 A and data not shown). PSD-95/SAP90 and S-SCAM were recruited into the Triton X-100-insoluble fraction, when coexpressed with MAGUIN-1 (Fig. 10, C and D). In contrast, PSD-95/SAP90 and S-SCAM remained in the Triton X-100-soluble fraction, when coexpressed with MAGUIN-2 (data not shown)." |
| Evidence tracking, Biological System: | Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) IP + WB/MSMS Y2H |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Shows co-localization of MAGUIN-1; Cnksr2 with PSD-95 (confocal). @ PMID:12390249 |
| SynGO annotation ID: | 3118 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |