| Annotated protein: | Dihydropyrimidinase-related protein 2 (DRP-2) (Collapsin response mediator protein 2) (CRMP-2) (Turned on after division 64 kDa protein) (TOAD-64). Gene symbol: DPYSL2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P47942 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DPYSL2 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | brain hippocampus spinal cord |
| Annotated paper: | Brittain JM, et al. "An atypical role for collapsin response mediator protein 2 (CRMP-2) in neurotransmitter release via interaction with presynaptic voltage-gated calcium channels" J Biol Chem. 2009 Nov 6;284(45):31375-90 PMID:19755421 |
| Figure(s): | Figs 2, 3 and 4, Fig.8a |
| Annotation description: | CRMP2 was shown to interact with CaV2.2 by coIP from synaptosome and hippocampal lysates, and by GST pulldown assays. Confocal microscopy using CRMP2 and CaV2.2 antibodies shows colocalisation of both proteins at synapses. 28/8/2017 Pim - Eperiments on localization are not conclusive in this paper to support term 'presynapse (GO:0098793)'. However, presynaptic annotation comes from: * Cav2.2 is a spcefically presynaptic Ca-channel. The authors seem to suggest that much in the Intro: "The N-type presynaptic Ca2+ channel (CaV2.2) is the predominant source of Ca2+ influx required for neurotransmitter release at immature CNS synapses (1)" * CRMP2 is target to the synapse (See Fig.8a, over-expression of GFP-tagged mouse CRMP2) * CRMP2 regulates SV release (Fig.8) * CRMP2 regulates Glutamate release via Ca2.2 (Fig.9) Together, I allow therefore annotation against term 'presynapse (GO:0098793)'. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS |
| Annotator(s): | John Chua (ORCID:0000-0002-5615-1014) |
| Lab: | Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Neurobiology/Ageing Program, Life Sciences Institute, National University of Singapore and Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore |
| Additional literature: | Immunostaining of CRMP2 with synaptophysin suggests synaptic localisation of CRMP2 in DRG neurons. @ PMID:19903690 |
| SynGO annotation ID: | 347 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |