| Annotated protein: | Synaptosomal-associated protein 25 (SNAP-25) (Super protein) (SUP) (Synaptosomal-associated 25 kDa protein). Gene symbol: SNAP25. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P60881 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SNAP25 |
| Ontology domain: | Cellular Component |
| SynGO term: | extrinsic component of presynaptic membrane (GO:0098888) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Hagiwara A, et al. "Differential distribution of release-related proteins in the hippocampal CA3 area as revealed by freeze-fracture replica labeling" J Comp Neurol. 2005 Aug 22;489(2):195-216 PMID:15983999 |
| Figure(s): | Fig 10 |
| Annotation description: | Co-labeling with CAST revealed distribution of the t-SNARE proteins syntaxin and synaptosomal-associated protein of 25 kDa (SNAP-25) in the AZ as well as in the extrasynaptic membrane surrounding the AZ (SZ). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Ruud Toonen (ORCID:0000-0002-9900-4233) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Shows that lipid anchor of SNAP-25 does not mediate its membran-association, but the interaction with syntaxin does. This ref justified annotation as "extrinsic component of ...." @ PMID:10644766 Figure 7 shows that SNAP-25 immunogold particles were present within and outside the active zones (Fig. 7a, b). Quantitative analysis revealed that the density of SNAP-25 in the active zone was very similar to that found on the non-synaptic part of the bouton (Fig. 7d) @ PMID:22683683 improved two-color fluorescence nanoscopy showed nanoscale clusters consisting of syntaxin-1 and SNAP-25 that contained associated Munc18-1 at presynaptic plasmamembrane 8/12/2016 Pim - Cortical neurons were used (Suppl info: " **Cortices** from newborn mouse pups were dissected in ice-cold Hank's Balanced Salt Solution ") @ PMID:23821748 |
| SynGO annotation ID: | 38 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |