| Annotated protein: | Voltage-dependent P/Q-type calcium channel subunit alpha-1A (Brain calcium channel I) (BI) (Calcium channel, L type, alpha-1 polypeptide, isoform 4) (Rat brain class A) (RBA-I) (Voltage-gated calcium channel subunit alpha Cav2.1). Gene symbol: CACNA1A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P54282 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CACNA1A |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic active zone membrane (GO:0099059) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Holderith N, et al. "Release probability of hippocampal glutamatergic terminals scales with the size of the active zone" Nat Neurosci. 2012 Jun 10;15(7):988-97 PMID:22683683 |
| Figure(s): | Fig 6 |
| Annotation description: | SDS-digested freeze-fracture replica-labeling (SDS-FRL) was used to visualize Cav2.1 in the stratum oriens of the hippocampal CA3 area at high resolution. Cav2.1 was enriched in presynaptic active zones compared to extrasynaptic bouton surfaces. Active zones were identified by clusters of intramembrane particles (IMP) as well co-labeling for the presynaptic active zone protein Rim1/2. Two Cav2.1 antibodies, one raised in a rabbit (Rb) and another one in a guinea pig (Gp) against different epitopes, labeled the same presynaptic active zone. These antibodies recognized intracellular epitopes on their target protein and thus were visualized by gold particles on the protoplasmic membrane half (P-face). Gold particles labeling the Cav2.1 subunit could not be found in Cav2.1-/- mice. 14/4/2017 Pim - "integral component of membrane ..." part is derived from the TM domains of Cav2.1. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548) |
| Lab: | Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA |
| Additional literature: | The distribution of Cav2.1 subunit of the P/Q-type channel was analyzed using highly sensitive SDS-digested freeze-fracture replica labeling in the rat cerebellar Purkinje cells. Localization is found in presynaptic parallel fiber terminals. The specificity of the immuno-gold labeling was confirmed in Cav2.1 KO synapses @ PMID:23426693 |
| SynGO annotation ID: | 384 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |