| Annotated protein: | AP-2 complex subunit sigma (Adaptor protein complex AP-2 subunit sigma) (Adaptor-related protein complex 2 subunit sigma) (Clathrin assembly protein 2 sigma small chain) (Clathrin coat assembly protein AP17) (Clathrin coat-associated protein AP17) (HA2 17 kDa subunit) (Plasma membrane adaptor AP-2 17 kDa protein) (Sigma2-adaptin). Gene symbol: AP2S1. Taxonomy: Homo sapiens (Human). Uniprot ID: P53680 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ AP2S1 |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle endocytosis (GO:0048488) |
| Annotated paper: | Morgan JR, et al. "A conserved clathrin assembly motif essential for synaptic vesicle endocytosis" J Neurosci. 2000 Dec 1;20(23):8667-76 PMID:11102472 |
| Figure(s): | fig. 8 and 9 |
| Annotation description: | rule: NAS_CPLX_2 rationale: The AP2 clathrin adapter is generally accepted to be a heterotetramer with subunit composition (AP2A1 OR AP2A2), AP2M1, AP2B1, AP2S1. Note that two gene paralogs are known for the alpha subunit (AP2A). Using an dominant negative peptide of the AP2B1 subunit the AP2 complex is shown to be involved in 'synaptic vesicle endocytosis (GO:0048488)'. Transfer of this annotation to the obligatory AP2M1 and AP2S1 subunitsis trivial based on the established protein composition of the AP2 complex. P_ref BP: [human AP2B1] SynGO ID 2739 (PMID:11102472, EM) P_inf CC: [AP2S1_MOUSE] SynGO ID 3937 (PMID:25211037, proteomics) Complex membership: AP2S1 protein is a fixed member of the AP2 clathrin adapter: - Shown in crystal structure (PMID:26960121, see Fig.1+2). Literal excerpt: "AP2 is a large (300kDa) heterotetrameric complex comprising large α and β2 subunits, a medium μ2 subunit and a small σ2 subunit." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Non-tracable Author Statement (NAS): | This annotation required some inference over additional information/literature, and is not solely based on observations made in the annotated paper by its original authors. |
| Annotator(s): | Peter McPherson (ORCID:0000-0001-7806-5662) |
| Lab: | Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada |
| SynGO annotation ID: | 3941 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |