| Annotated protein: | Triple functional domain protein (EC 2.7.11.1) (PTPRF-interacting protein). Gene symbol: TRIO. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: F1M0Z1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ TRIO |
| Ontology domain: | Biological Process |
| SynGO term: | modulation of chemical synaptic transmission (GO:0050804) |
| Synapse type(s): | Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Herring BE, et al. "Kalirin and Trio proteins serve critical roles in excitatory synaptic transmission and LTP" Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):2264-9 PMID:26858404 |
| Figure(s): | Fig 1 and 2 |
| Annotation description: | Fig1: overexpressed Trio-9, the trio isoform most abundant in the hippocampus increased synaptic AMPAR current amplitude (but not NMDAR) in CA1 pyr. neurons. Fig2: shRNA-mediated Trio depletion reduces AMPAR current amplitude (but not NMDAR). This phenotype is nearly identical to modulation of Trio's paralog Kalirin. Depletion of both proteins leads to 80% reduction of spines and severely affected AMPAR and NMDAR current amplitudes. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Ruud Toonen (ORCID:0000-0002-9900-4233) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | In apparent contrast to Herring et al, this paper shows that Trio shRNA-mediated depletion increases neurite numbers and complexity in cultured rat hippocampal neurons at 7 DIV. In acute hippocampal slices Trio shRNA resulted in increased AMPAr-, but not NMDAr-mediated EPSCs. @ PMID:26721934 |
| SynGO annotation ID: | 3948 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |