Annotated protein:	Regulating synaptic membrane exocytosis protein 1 (Rab-3-interacting molecule 1) (RIM 1) (Rab-3-interacting protein 1). Gene symbol: RIMS1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q99NE5
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SynGO gene info:	SynGO data @ RIMS1
Ontology domain:	Biological Process
SynGO term:	structural constituent of active zone (GO:0098882)
Synapse type(s):	hippocampus
Annotated paper:	Wang SSH, et al. "Fusion Competent Synaptic Vesicles Persist upon Active Zone Disruption and Loss of Vesicle Docking" Neuron. 2016 Aug 17;91(4):777-791 PMID:27537483
Figure(s):	Figure 1, 2, 3, 4, 5, 7
Annotation description:	It is demonstrated that RIM1 is required to maintain the structural integrity of the active zone. Genetic removal of active zone proteins RIM1, RIM2, ELKS1 and ELKS2 leads to a loss of Bassoon, Munc13 and Piccolo, and a reduction of RIMBP2 and Cav2.1 (Figure 1), thus showing a severe reduction of the active zone. In line with disruption of the active zone, multiple essential functions of the presynaptic terminal are affected, including vesicle docking (figure 2), vesicle priming (figure 7), spontaneous and action potential induced fusion (figure 3 and 5) and calcium influx (figure 4). Removal of RIM1/2 or ELKS1/2 alone is not sufficient to disrupt the active zone (figure 7). 10/4/2017 Pim - I have added the link to SynGO ID #60 that shows that RIM1 is itself localized to the AZ. I think this strengthens the selected term.
Evidence tracking, Biological System:	Cultured neurons
Evidence tracking, Protein Targeting:	Genetic transformation (eg; knockout, knockin, mutations)
Evidence tracking, Experiment Assay:	Electron Microscopy Confocal Whole-cell patch clamp Western blot
Annotator(s):	Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958)
Lab:	Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA
SynGO annotation ID:	400
Dataset release (version):	20231201
View annotation as GO-CAM model: