| Annotated protein: | Glutamate receptor ionotropic, delta-1 (GluD1) (GluR delta-1 subunit). Gene symbol: GRID1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q61627 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GRID1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | neocortex, GABAergic |
| Annotated paper: | Fossati M, et al. "Trans-Synaptic Signaling through the Glutamate Receptor Delta-1 Mediates Inhibitory Synapse Formation in Cortical Pyramidal Neurons" Neuron. 2019 Dec 18;104(6):1081-1094.e7 PMID:31704028 |
| Figure(s): | Fig 3B |
| Annotation description: | Fig3B: Using Electron Microscopy, immunogold labelled GluD1 showed post-synaptic localisation opposite to inhibitory pre-synapse labelled by anti-vGAT staining using DAB (diaminobenzidine) labelling. GluD1 was detected in the post-synapse in a region lateral to the active zone and also in intracellular compartments. The EM was done in layer2/3 cortex of P21 mice. (Fig S1A: The antibody was validated by western blotting, where the signal was not seen in cultured neurons treated with GluD1 shRNA.) |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Carlos Pascual-Caro (ORCID:0000-0002-2345-393X) Anjali Amrapali Vishwanath (ORCID:0000-0002-9888-0928) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) |
| Lab: | ICM - Brain and Spine Institute, Hpital Piti Salptrire, 47 Boulevard de l'Hpital, 75013 Paris, France |
| Additional literature: | This paper uses Cryo-EM to determine the structure of rat GluD1, demonstrating that it has transmembrane, intracellular and extracellular domain. Hence, the protein is an integral part of the membrane. @ PMID:31925409 |
| SynGO annotation ID: | 4013 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |