| Annotated protein: | Regulating synaptic membrane exocytosis protein 1 (Rab-3-interacting molecule 1) (RIM 1) (Rab-3-interacting protein 1). Gene symbol: RIMS1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q99NE5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RIMS1 |
| Ontology domain: | Biological Process |
| SynGO term: | presynaptic dense core vesicle exocytosis (GO:0099525) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Persoon CM, et al. "The RAB3-RIM Pathway Is Essential for the Release of Neuromodulators" Neuron. 2019 Dec 18;104(6):1065-1080.e12 PMID:31679900 |
| Figure(s): | Figure 2, S3 and S6 |
| Annotation description: | Figure 2C-F: RIM1/2 cDKO neurons showed more than 95% reduction in NPY-mCherry-labeled DCV fusion events upon stimulation compared to WT. Figure S3 C-G: The extrasynaptic events in RIM cDKO neurons lasted within 1 s (S3 E, indicated by a red bar), and were not detected as NPY-pHluorin-labeled fusion events. Figure S3 H: The total number of NPY-mCherry puncta was not altered between the genotypes. Figure S3 J-L: The loss of DCV fusion in RIM cDKO neurons was confirmed using BDNF-pHluorin as an independent reporter for neuromodulator release. Figure S6A-F: DCV fusion was strongly reduced in RIM1-deficient neurons (RIM1-/-, RIM2+/+ and RIM1-/-, RIM2+/-) and RIM2-deficient neurons heterozygous for RIM1 further denoting the role of RIM1 in the regulation of presynaptic dense-core vesicle exocytosis. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | pHluorin assay |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4028 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |