Annotated protein:Acetylcholine regulator unc-18 (Uncoordinated protein 18). Gene symbol: UNC-18. Taxonomy: Caenorhabditis elegans (Worm). Uniprot ID: P34815
antibody wiki:
SynGO gene info:SynGO data @ STXBP1
Ontology domain:Biological Process
SynGO term:presynaptic dense core vesicle exocytosis (GO:0099525)
Synapse type(s):ganglion
Annotated paper:Zhou KM, et al. "PKA activation bypasses the requirement for UNC-31 in the docking of dense core vesicles from C. elegans neurons" Neuron. 2007 Nov 21;56(4):657-69 PMID:18031683
Figure(s):Figure 2
Annotation description:This paper introduces a new technique to measure DCV exocytosis kinetics using high-resolution membrane capacitance (Cm) combined with Ca2+ uncaging stimuli.

Figure 2A: Average Cm responses (bottom) induced by similar [Ca2+]i elevations (top) in wild-type (black trace), unc-31 mutant (red trace), unc-64 mutant (blue trace), and unc-18 mutant (grey trace) cells are compared. The inset compares the amplitude of burst exocytosis between wild-type, unc-31, unc-64, unc-18, and tag-81 mutant cells. The exocytotic burst is quantified as the amplitude of the exponential fit to the Cm trace.
Drastic reduction (~85%) in the amplitude of burst exocytosis of the Unc18 mutant indicates its role in DCV exocytosis.

Figure 7A: Density of near-membrane DCVs per unit area was slightly smaller in unc-18(e81) neurons.
Figure 7B: The average docking rate per unit area per minute was considerably reduced (by ~58%) in unc-18(e81) mutant cells.
Figure 7C: Docking events in the absence of UNC-18 were in general much shorter than those from wild-type cells.

Together, the data suggest that Unc18 plays a key role in presynaptic DCV exocytosis.
Evidence tracking, Biological System:Cultured neurons
Evidence tracking, Protein Targeting:Genetic transformation (eg; knockout, knockin, mutations)
Evidence tracking, Experiment Assay:Microscopy (generic)
Whole-cell patch clamp
Annotator(s):Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:4072
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology