| Annotated protein: | Acetylcholine regulator unc-18 (Uncoordinated protein 18). Gene symbol: UNC-18. Taxonomy: Caenorhabditis elegans (Worm). Uniprot ID: P34815 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ STXBP1 |
| Ontology domain: | Biological Process |
| SynGO term: | presynaptic dense core vesicle exocytosis (GO:0099525) |
| Synapse type(s): | ganglion |
| Annotated paper: | Zhou KM, et al. "PKA activation bypasses the requirement for UNC-31 in the docking of dense core vesicles from C. elegans neurons" Neuron. 2007 Nov 21;56(4):657-69 PMID:18031683 |
| Figure(s): | Figure 2 |
| Annotation description: | This paper introduces a new technique to measure DCV exocytosis kinetics using high-resolution membrane capacitance (Cm) combined with Ca2+ uncaging stimuli. Figure 2A: Average Cm responses (bottom) induced by similar [Ca2+]i elevations (top) in wild-type (black trace), unc-31 mutant (red trace), unc-64 mutant (blue trace), and unc-18 mutant (grey trace) cells are compared. The inset compares the amplitude of burst exocytosis between wild-type, unc-31, unc-64, unc-18, and tag-81 mutant cells. The exocytotic burst is quantified as the amplitude of the exponential fit to the Cm trace. Drastic reduction (~85%) in the amplitude of burst exocytosis of the Unc18 mutant indicates its role in DCV exocytosis. Figure 7A: Density of near-membrane DCVs per unit area was slightly smaller in unc-18(e81) neurons. Figure 7B: The average docking rate per unit area per minute was considerably reduced (by ~58%) in unc-18(e81) mutant cells. Figure 7C: Docking events in the absence of UNC-18 were in general much shorter than those from wild-type cells. Together, the data suggest that Unc18 plays a key role in presynaptic DCV exocytosis. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Microscopy (generic) Whole-cell patch clamp |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4072 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |