| Annotated protein: | Leucine-rich repeat and fibronectin type-III domain-containing protein 2. Gene symbol: LRFN2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q80TG9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ LRFN2 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Brouwer M, et al. "SALM1 controls synapse development by promoting F-actin/PIP2-dependent Neurexin clustering" EMBO J. 2019 Sep 2;38(17):e101289 PMID:31368584 |
| Figure(s): | Fig2 |
| Annotation description: | Fig 2A-B: Immunocytochemical study demonstrates colocalisation (~90%) of endogenous SALM1 (green) with presynaptic excitatory synapse marker VGluT1 (red). FigC: Higher magnification indicates SALM1 puncta fully overlapping with presynaptic VGluT1 puncta. Fig D-E: Electron micrographs showing subsynaptic localization of endogenous SALM1 in mouse hippocampal brain slices. SALM1 immunogold particles are detected in presynapses (indicated by orange and magenta arrows) and were mostly detected in presynaptic terminals (~60%). Figure S1B-D : Western blot showing lysates of HEK cells transfected with empty vector (control), SALM1-mCherry or SALM2, 3, 4 or 5-FLAG were stained for anti-SALM1. The SALM1 antibodies specifically recognize SALM1-mCherry. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4093 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |