| Annotated protein: | Early endosome antigen 1. Gene symbol: EEA1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8BL66 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EEA1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic early endosome (GO:0098842) |
| Synapse type(s): | Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Selak S, et al. "Human autoantibodies against early endosome antigen-1 enhance excitatory synaptic transmission" Neuroscience. 2006 Dec 28;143(4):953-64 PMID:17113235 |
| Figure(s): | Fig2 and 3 |
| Annotation description: | Fig2A-B: Human anti-EEA1 (blue) and mouse anti-synaptophysin antibodies (yellow) display distinct immunoreactivity in the CA3 field of the hippocampus. Antibodies against early endosome marker protein EEA1 produce a characteristic vesicular staining pattern in CA3 cell bodies and dendrites and do not co-localize with synaptophysin antibodies, which label the presynaptic nerve terminals but rather are in close proximity of Synaptophysin puncta (inset). Fig 2C: Majority of EEA1 puncta (blue) colocalise with PSD-95 (yellow) on cultured hippocampal neurons. Figure 1: Independent antibody validation was performed to verify the specificity of the human-anti-EEA1 used in the study. Confocal images showed the co-localization of human anti-EEA1 (blue) and mouse monoclonal (yellow) anti-EEA1 antibodies in cultured COS-7 cells. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4115 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |