| Annotated protein: | PTPRF interacting protein alpha 1. Gene symbol: PPFIA1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: D3ZZ81 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PPFIA1 |
| Ontology domain: | Cellular Component |
| SynGO term: | spine apparatus (GO:0097444) |
| Synapse type(s): | hippocampus Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Hoogenraad CC, et al. "Liprinalpha1 degradation by calcium/calmodulin-dependent protein kinase II regulates LAR receptor tyrosine phosphatase distribution and dendrite development" Dev Cell. 2007 Apr;12(4):587-602 PMID:17419996 |
| Figure(s): | Fig. 1 |
| Annotation description: | Cultured hippocampal neurons (17 days in vitro; DIV17) were immunostained with antibodies against liprina1 and CamKIIa and imaged using confocal microscopy. A punctate pattern of Liprin-alpha 1 was shown as previously described (Ko et al., 2003; Shin et al., 2003; Wyszynski et al., 2002), co- localizing with CaMKIIa in dendritic spines (Figure 1A). Liprin-alpha 1 puncta also showed extensive overlap with PSD-95, a postsynaptic density protein, and with bassoon, a pre- synaptic active zone protein (Figure 1B), indicating the presence of Liprin-alpha 1 at synapses. The intensity of liprina staining was generally weaker and more variable than the staining of these synaptic markers, such that some synapses had robust liprina staining while other synapses showed undetectable signal (Figures 1A and 1B). Quantification revealed that |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| Additional literature: | Rabbit Liprin alpha 1 antibody (1069) was generated against the rat peptide sequence: SFRRAPSWRKKFRPKDIR and affinity purified. Confocal microscopy was used to demonstrate immunolocalization of Liprin-alpha 1 with postsynaptic proteins GRIP and LAR-RPTP (Fig. 3) @ PMID:11931740 Rabbit Liprin alpha 1 antibody (1069) was generated against the mouse peptide sequence: CSHGSGSPSQPDADSHFE and affinity purified. Confocal microscopy images of immunocytochemistry of mouse primary cortical neurons DIV 14 colabeled with antibodies against Liprin-alpha 1 and PSD-95 show colocalization (Fig. 12) @ PMID:21618221 At murine neuromuscular junctions, confocal microscopy demonstrated localization of Liprin-alpha 1 to Z-discs, alpha-actinin and AChRs in skeletal muscles indicating postsynaptic localization @ PMID:28831123 |
| SynGO annotation ID: | 4173 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |