| Annotated protein: | PTPRF interacting protein alpha 1. Gene symbol: PPFIA1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A0A0G2K3L9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PPFIA1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic membrane neurotransmitter receptor levels (GO:0099072) |
| Synapse type(s): | hippocampus Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Wyszynski M, et al. "Interaction between GRIP and liprin-alpha/SYD2 is required for AMPA receptor targeting" Neuron. 2002 Mar 28;34(1):39-52 PMID:11931740 |
| Figure(s): | Fig. 6,7 |
| Annotation description: | Fig. 6: Liprin-alpha 1 overexpression was shown to disrupt dendritic clustering and surface expression of AMPA receptors. Cultured hippocampal neurons were transfected at DIV14 with either liprin-alpha-1A or liprin-alpha 1, and doubled labeled at DIV20 for overexpressed liprin-alpha 1 and endogenous GluR2/3, endogenous PSD-95, endogenous surface GluR1, or endogenous surface GluR2. Transfected neurons were detected using anti- liprin- alpha 1 antibodies at a low dilution that allowed visualization of cells overexpressing liprin-alpha 1, but not of endogenous liprin- alpha1in untransfected cells. Fig. 7: Liprin-alpha1A was shown to inhibit surface expression of GluR2 but Not GluR1. The same methodology of Fig. 6 was used with cells triple labeled for overexpressed liprin-alpha1 or liprin-alpha1A, surface HA-GluR1 or surface HA-GluR2, and intracellular HA-GluR1 or HA-GluR2. The mammalian expression constructs for liprin-alpha 1 and liprin-alpha 1A and the mouse monoclonal liprin-alpha 1 antibody (1.77) were used from Serra-Pages et al., 1995. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| SynGO annotation ID: | 4186 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |