| Annotated protein: | Contactin-associated protein-like 2 (Cell recognition molecule Caspr2). Gene symbol: CNTNAP2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9CPW0 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CNTNAP2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic membrane neurotransmitter receptor levels (GO:0099072) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Varea O, et al. "Synaptic abnormalities and cytoplasmic glutamate receptor aggregates in contactin associated protein-like 2/Caspr2 knockout neurons" Proc Natl Acad Sci U S A. 2015 May 12;112(19):6176-81 PMID:25918374 |
| Figure(s): | Figures 3 and 4 |
| Annotation description: | Cultured cortical pyramidal neurons from Cntnap2-/- mice show decreases in dendritic spine density and GluA1 (AMPAR subunit) contents in spines (Figure 3) and an increased cytoplasmic aggregates containing GluA1 (Figure 4), suggesting that Cntnap2 promotes synaptic localization of GluA1. Figure 3 and 4 legends Fig. 3. Alterations in spine morphology and GluA1 content in spines in cultured cortical pyramidal neurons from Cntnap2-/- mice. (D) Quantification of average GluA1 cluster immunofluorescence intensity/area in spines and shaft. Fig. 4. Cytoplasmic aggregates containing GluA1 are present in Cntnap2-/- cortical pyramidal neurons. ..... (B) Quantification of the percent of cells containing GluA1 positive aggregates in the soma in wild-type and Cntnap2-/- neurons. 18/01/2021 Dnyanada: The paper demonstrates that Cntnap2 knock-out does not affect synapse formation, as measured by the number of PSD95-synapsin clusters (Figure 2). On the other hand, it shows that Cntnap2 knock-out results in a decrease in spine density, specifically a reduction in total GluA1 content in the spines (Figure 3) in mature synapses. Cntnap2 knock-out neurons also demonstrated the presence of large cellular aggregates of GluA1 (Figure 4) indicating the reduction in surface GluA1 receptors might be due to impaired trafficking. Based on this, the term "regulation of postsynaptic membrane neurotransmitter receptor levels" was chosen. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal Microscopy (generic) |
| Annotator(s): | Hwajin Jung (ORCID:0000-0001-6194-9648) Eunjoon Kim (ORCID:0000-0001-5518-6584) |
| Lab: | Center for Synaptic Brain Dysfunctions, Institute for Basic Science (IBS) and Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, South Korea |
| SynGO annotation ID: | 4191 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |