Annotated protein:Cyclin-dependent kinase-like 5. Gene symbol: CDKL5. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: D3ZG85
antibody wiki:
SynGO gene info:SynGO data @ CDKL5
Ontology domain:Cellular Component
SynGO term:postsynaptic density, intracellular component (GO:0099092)
Synapse type(s):cerebral cortex, glutamatergic
Annotated paper:Zhu YC, et al. "Palmitoylation-dependent CDKL5-PSD-95 interaction regulates synaptic targeting of CDKL5 and dendritic spine development" Proc Natl Acad Sci U S A. 2013 May 28;110(22):9118-23 PMID:23671101
Figure(s):Fig. 1, 3, S1, S2
Annotation description:Fig. 1 colocalization of CDKL5 with PSD-95, coimmunoprecipitation of CDKL5 with PSD-95 but not with Synaptophysin.
Fig. 3 localization of CDKL5 to synapses depend on PSD-95
Fig. S2 CDKL5 is enriched in the PSD fraction

14/4/2017 Pim
Fig.S1 (binding of recombinant PSD-95 and CDKL5) shows that the interaction of these proteins is direct. This places CDKL5 in the same cellular compartment as PSD-95 --> postsynapse.
Evidence tracking, Biological System:Intact tissue
Cultured neurons
Cell-free system
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Confocal
Biochemical fractionation (generic)
IP + WB/MSMS
Protein-protein interaction (generic)
Annotator(s):Chiara Verpelli (ORCID:0000-0003-2949-9725)
Carlo Sala (ORCID:0000-0003-0662-9523)
Lab:CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy
SynGO annotation ID:420
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology