Annotated protein:Contactin-associated protein-like 4 (Cell recognition molecule Caspr4). Gene symbol: CNTNAP4. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q99P47
antibody wiki:
SynGO gene info:SynGO data @ CNTNAP4
Ontology domain:Cellular Component
SynGO term:integral component of presynaptic membrane (GO:0099056)
Synapse type(s):cerebral cortex, GABAergic
Annotated paper:Karayannis T, et al. "Cntnap4 differentially contributes to GABAergic and dopaminergic synaptic transmission" Nature. 2014 Jul 10;511(7508):236-40 PMID:24870235
Figure(s):Fig. 1
Annotation description:Fig. 1: Cntnap4 is present at presynaptic fraction, Fc-tagged Cntnap4 binds to soma and proximal dendrites, apposed to gephyrin puncta.

13/4/2017 Pim
- Cntnap4 has a TM domain. The Fc-tagged Cntnap4 construct is the extracellular domain of this protein. Fig.1 suggests that Cntnap4 is a presynaptic TM protein that interacts with proteins attached to gephyrin-positive postsynaptic elements. For this to occur the protein must be positioned in the presynaptic plasma membrane. This is not directly shown, but implied in the paper.
- "Integral component ..." is inferred form the TM domain of Cntnap4.
Evidence tracking, Biological System:Intact tissue
Cultured neurons
Evidence tracking, Protein Targeting:Over-expression
Antibody (detection)
Evidence tracking, Experiment Assay:Wide-field fluorescence
Western blot
Biochemical fractionation (generic)
Annotator(s):Chiara Verpelli (ORCID:0000-0003-2949-9725)
Carlo Sala (ORCID:0000-0003-0662-9523)
Lab:CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy
SynGO annotation ID:423
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology