| Annotated protein: | Ephrin type-B receptor 2 (EC 2.7.10.1) (Neural kinase) (Nuk receptor tyrosine kinase) (Tyrosine-protein kinase receptor EPH-3) (Tyrosine-protein kinase receptor SEK-3) [Cleaved into: EphB2/CTF1; EphB2/CTF2]. Gene symbol: EPHB2. Taxonomy: Mus musculus (Mouse). Uniprot ID: P54763 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EPHB2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | hippocampus Mossy fiber synapse (DG->CA3) |
| Annotated paper: | Liu XD, et al. "Retrograde regulation of mossy fiber axon targeting and terminal maturation via postsynaptic Lnx1" J Cell Biol. 2018 Nov 5;217(11):4007-4024 PMID:30185604 |
| Figure(s): | Fig 4B and Fig 4E |
| Annotation description: | EphB2 is a transmembrane protein of the plasma membrane. In figure 4B, the authors "...purified the synaptosomes of hippocampal tissues and found that Lnx1 could be pulled down by EphB2 antibodies from PSD but not non-PSD fractions...". Thus, antibodies against EphB2 immunoprecipitate the endogenous protein from synaptic fractions. EphB2 is not itself a component of the post-synaptic density (PSD) but interacts with a protein that is at the PSD. In figure 4E, the authors demonstrate "...colocalization of Lnx1 and EphB2 in CA3 pyramidal neurons, immunostaining was used in hippocampal sections and superresolution imaging with stimulated emission depletion (STED) techniques were performed to visualize the localization of these proteins. The STED imaging resolved discrete puncta containing both Lnx1 and EphB2 around the membrane of CA3 neurons". Thus, antibodies against EphB2 co-localize on the membrane of CA3 neurons with a known component of the post-synapse. 18/01/2021 Dnyanada: P54763 (EPHB2_MOUSE) demonstrates the transmembrane and topological domains of the EPHB2 protein. SynGO ID: 256 also annotates EphB2 to the term "integral component of postsynaptic membrane" using immunoEM data. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Super resolution Biochemical fractionation (generic) IP + WB/MSMS |
| Annotator(s): | Peter McPherson (ORCID:0000-0001-7806-5662) |
| Lab: | Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada |
| SynGO annotation ID: | 4507 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |